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mutant atcc 27853 16b1  (ATCC)


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    Structured Review

    ATCC mutant atcc 27853 16b1
    Plate showing the effects of different pyoverdines on the killing of P. aeruginosa <t>ATCC</t> <t>27853</t> by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.
    Mutant Atcc 27853 16b1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22791 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+atcc+27853+16b1/Pseudomonas+aeruginosa+(Schroeter)+Migula/pmc00093868-114-92-93
    Average 99 stars, based on 22791 article reviews
    mutant atcc 27853 16b1 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa"

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa

    Journal:

    doi:

    Plate showing the effects of different pyoverdines on the killing of P. aeruginosa ATCC 27853 by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.
    Figure Legend Snippet: Plate showing the effects of different pyoverdines on the killing of P. aeruginosa ATCC 27853 by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.

    Techniques Used: Purification

    Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853
    Figure Legend Snippet: Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853

    Techniques Used:

    Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).
    Figure Legend Snippet: Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).

    Techniques Used: Electrophoresis, Mutagenesis

    Related Articles

    Electrophoresis:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa
    Article Snippet: .. Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). .. Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).

    Membrane:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa
    Article Snippet: .. Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). .. Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).

    Mutagenesis:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa
    Article Snippet: .. Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). .. Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).



    Similar Products

    mutant atcc 27853 16b1  (ATCC)
    99
    ATCC mutant atcc 27853 16b1
    Plate showing the effects of different pyoverdines on the killing of P. aeruginosa <t>ATCC</t> <t>27853</t> by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.
    Mutant Atcc 27853 16b1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+atcc+27853+16b1/Pseudomonas+aeruginosa+(Schroeter)+Migula/pmc00093868-114-92-93
    Average 99 stars, based on 1 article reviews
    mutant atcc 27853 16b1 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Plate showing the effects of different pyoverdines on the killing of P. aeruginosa ATCC 27853 by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.

    Journal:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa

    doi:

    Figure Lengend Snippet: Plate showing the effects of different pyoverdines on the killing of P. aeruginosa ATCC 27853 by pyocin S3. To a lawn of P. aeruginosa ATCC 27853 on Luria-Bertani medium drops of purified pyocin S3 (20 μl, corresponding to 25 U ml−1, defined as the reciprocal of the highest pyocin dilution inhibiting bacterial growth) were deposited at three places in the middle. Next, on each side of the pyocin drop, 20 μl of the following purified pyoverdines (2 μmol) was added: that from PAO1 (top), that from ATCC 27853 (middle), and that from clinical isolate PA6 (bottom). Only the cognate pyoverdine (ATCC 27853), recognized by the pyoverdine receptor, competes with pyocin S3, which uses the same receptor, inhibiting the killing.

    Article Snippet: Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6).

    Techniques: Purification

    Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853

    Journal:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa

    doi:

    Figure Lengend Snippet: Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853

    Article Snippet: Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6).

    Techniques:

    Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).

    Journal:

    Article Title: Uptake of Pyocin S3 Occurs through the Outer Membrane Ferripyoverdine Type II Receptor of Pseudomonas aeruginosa

    doi:

    Figure Lengend Snippet: Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6). Lane 7 contains the molecular mass markers (High molecular mass markers; Pharmacia-LKB, Uppsala, Sweden).

    Article Snippet: Pyoverdine production, growth stimulation by pyoverdine, and uptake of ferripyoverdine of wild-type and pyocin S3-susceptible and -resistant mutants of strains Pb60 and ATCC 27853 fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Sodium dodecyl sulfate-polyacrylamide gel (7%) electrophoresis of 10-μg quantities of outer membrane proteins from P. aeruginosa ATCC 27853 grown in CAA medium (lane 1) and in CAA medium plus FeCl 3 (50 μM) (lane 2), mutant ATCC 27853-5B (lane 3), mutant ATCC 27853-6A (lane 4), mutant ATCC 27853-9A1 (lane 5), and mutant ATCC 27853-16B1 (lane 6).

    Techniques: Electrophoresis, Mutagenesis